曝光Marker
| 货号. | 品名 | 规格 | 价格 | |
|---|---|---|---|---|
| M201 | 曝光Marker | 100ul | RMB 400.00 | |
| M202 | 曝光Marker | 250ul | RMB 800.00 | |
| M210 | 曝光Marker | 1250ul | RMB 3200.00 |
Exposure Marker条带能够与IgG恒定区结合，因此可以在X胶片上曝光显色。
曝光Marker使用：
1. 室温下解冻后轻轻混匀或者是用移液枪缓慢吹打均匀；
2. 取本品1-5μl与实验样品同时进行聚丙烯酰胺凝胶电泳；
3. 电泳结束后根据标准的实验步骤进行Western Blot实验；
4. 使用ECL工作液在暗室中将信号曝光到X光胶片上。
Introduction:
Exposure Marker（Western Blot Marker） is designed for facility western blot, which can conveniently monitors the electrophoresis progress and directly visualize protein standard bands on a blot. It consists of eight recombinant proteins in the range of 12-90 KDa (12、17、23、30、38、50、70、90 KDa). One of them, the 70 kDa protein coupled with a purple dye forms a pre-stained protein which is used for detection the electrophoresis and evaluation western transfer efficiency. The other seven proteins can band with human, mouse, rat, goat, sheep and rabbit serum with their IgG banding site, therefore they can directly band with secondary antibody, or primary antibody firstly then the secondary antibody. After detection, users can observe standard protein bands on the X-ray as shown in Fig.1. Meanwhile they can read the M.W. of positive signal according to the position of the standard protein bands.
Storage Buffer:
125 mM Tris-HCl, pH 6.8, 10 mM DTT, 2 mM EDTA, 2 % (W/V) SDS, 10 % (W/V) Glycerol, 0.01 % (W/V) Bromophenol blue.
Directions for Use:
1. Thaw at room temperature, blend gently to make it well-distributed.
2. Load 5-10μl uMarkerTM Western Blot Marker in a well of one prepared SDS-PAGE gel, meanwhile load your samples in the other wells and perform the electrophoresis.We recommend testing different amounts of the standards to determine the optimal amount of standards to use under your experimental conditions.
3. Transfer proteins to a NC or PVDF membrane.
4. Perform the blocking step, incubating with primary antibody and secondary antibody.
5. After the fully washing, make the membrane reaction with ECL reagent, then exposure the X-ray film in the dark room.
Example:
